Being a quite chemically and biologically inert amino acid, asparagine is used as a nitrogen storage and transport molecule in many plants. It is, in fact, the largest nitrogen transporter in both the xylem and the phloem. In the fixation of nitrogen in legumes, asparagine is the highest final product and it accumulates in high levels in the nodules and exudates of the root’s xylem. Asparagine accumulates intensely in germinating seeds, thus being considered as the most important amino acid, quantitatively speaking, in the germination process. Numerous studies also show the accumulation of this amino acid in drought-induces stress, phosphorus and sulfur deficiencies or in heavy metal poisoning.